Correlation between the sperm characteristics evaluated by a computerized system (CASA) and the reproductive performance of sows

Emanuelle Maria Gottardi, Thaisa Campos Marques, Karen Martins Leão, Francisco Ribeiro de Araújo Neto, Leidiane Gonçalves Fernandes

Abstract


Computer-assisted semen analysis (CASA) systems have been one the most used tools to evaluate sperm kinetics. The objective of this research was to estimate the correlation between sperm motility characteristics evaluated by CASA during 72 hours of cooling with the farrowing rate (FR) and total number of piglets born (TNB) after artificial insemination. Multiparous sows (n=464) were inseminated with semen from seven boars (19.6 ± 1.3 ejaculates/male). Sperm motility parameters were determined immediately after dilution and after 24, 48 and 72 hours of cooling at 15°C: total motility (TM-%), progressive motility (PM-%), curvilinear velocity (VCL-μm/s), straight line velocity (VSL-μm/s), average path velocity (VAP-µm/s), amplitude of lateral head displacement (ALH-µm), flagellar beat cross frequency (BCF-Hz), straightness (STR-%) and linearity (LIN-%). Pearson’s correlation coefficient was applied to analyze the data and the comparison of the means of the sperm characteristics between the boars was done by Tukey’s test at 5% probability. TM and PM at time zero (T0) were significant and had a moderate to high correlation with FR and TNB. After 72 hours of refrigeration, the semen quality was reduced and showed a significant and low correlation of the TM and PM with these same parameters. The boar presenting the lowest value of TM and PM after dilution obtained lower FR and TNB. In conclusion, computer-assisted semen analysis soon after dilution can be used to predict fertility of boars.


Keywords


Farrowing rate; Fertility; Sperm movement; Total born piglets.

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References


Alkmin, D. V., Parrilla, I., Tarantini, T., Parlapan, L., Del Olmo, D., Vazquez, J. M. & Martinez, E. A., Roca, J. (2014). Intra-and interboar variability in flow cytometric sperm sex sorting. Theriogenology, 82 (3), 501-508. doi: 10.1016/j.theriogenology.2014.05.008.

Bennemann, P. E., Diehl, G. N., Milbradt, E., Vidor, R. M., Fries, H. C. C., Wentz, I., Bernardi, M. L. & Bortolozzo, F. P. (2005). Artificial insemination of gilts with 1.5 billion sperms stored in different periods associated with different pre-ovulatory intervals. Reproduction in Domestic Animals, 40 (6), 507–510. doi: 10.1111/j.1439-0531.2005.00614.x.

Broekhuijse, M. L. W. J., Gaustad, A. H., Bolarin Guillén, A. & Knol, E. F. (2015). Efficient Boar Semen Production and Genetic Contribution: The Impact of Low‐Dose Artificial Insemination on Fertility. Reproduction in Domestic Animals, 50 (S2), 103-109. doi: 10.1111/rda.12558.

Broekhuijse, M. L. W. J., Sostaric, E., Feitsma, H. & Gadella, B. M. (2012). The value of microscopic semen motility assessment at collection for a commercial artificial insemination center, a retrospective study on factors explaining variation in pig fertility. Theriogenology, 77 (7), 1466-1479. doi: 10.1016/j.theriogenology.2011.11.016.

Cox, J. F., Alfaro, V., Montenegro, V. & Rodriguez-Martinez, H. (2006). Computer-assisted analysis of sperm motion in goats and its relationship with sperm migration in cervical mucus. Theriogenology, 66 (4), 860-867. doi: 10.1016/j.theriogenology.2006.01.062.

Dyck, M. K., Foxcroft, G. R., Novak, S., Ruiz-Sánchez, A., Patterson, J. & Dixon, W. T. (2011). Biological markers of boar fertility. Reproduction in Domestic Animals, 46 (S2), 55-58. doi: 10.1111/j.1439-0531.2011.01837.x.

Ferreira, C. E. R., Savio, D. B., Guarise, A. C., Flach, M. J., Gastal, G. D. A., Gonçalves, A. O., Dellagostin, O. A., Alonso, R. V., Bianchi, I., Corcini, C. D. & Lucia, T. (2015). Contribution of boars to reproductive performance and paternity after homospermic and heterospermic artificial insemination. Reproduction, Fertility and Development, 27 (7), 1012-1019. doi: 10.1071/RD13418.

Foxcroft, G. R., Patterson, J., Cameron, A. & Dyck, M. K. (2010, July). Application of advanced AI technologies to improve the competitiveness of the pork industry. Proceedings of 21st International Pig Veterinary Society Congress, Vancouver, BC Canada. Retrieved May 20, 2019, from https://orbit.dtu.dk/fedora/objects/orbit:63423/datastreams/file_5574380/content.

Gerhardt, T. E. & Silveira, D. T. (2009). Métodos de pesquisa. Porto Alegre, Brasil: Editora da UFRGS. Retrieved Apr 4, 2020, from http://www.ufrgs.br/cursopgdr/downloadsSerie/derad005.pdf.

Gil, A. C. (2017). Como Elaborar Projetos de Pesquisa. São Paulo, Brasil: Editora Atlas. 192p.

Iida, R. & Koketsu, Y. (2016). Lower farrowing rate in female pigs associated with higher outdoor temperatures in humid subtropical and continental climate zones in Japan. Animal Reproduction, 13 (2), 63-68. doi: 10.21451/1984-3143-AR728.

Kadirvel, G., Kalita, M. K., Dewry, R. K., Kumar, A., Mahanta, N., Singh, L. A., Baishya, S. K. & Ngachan, S. V. (2019). Semen quality and fertility of liquid stored and frozen-thawed semen in crossbred pigs of North-Eastern India. Indian Journal of Animal Research, 53 (1), 41-44. doi: 10.18805/ijar.B-3464.

Kemp, B. & Soede N. M. (1996). Relationship of weaning-to estrus interval to timing of ovulation and fertilization in sows. Journal of Animal Science, 74 (5), 944–949. doi: 10.2527/1996.745944x.

Knox, R.V. & Rodriguez-Zas, S. (2001). Factors influencing estrus and ovulation in weaned sows as determined by transrectal ultrasound. Journal of Animal Science, 79 (12), 2957–2963. doi: 10.2527/2001.79122957x.

Kommisrud, E., Paulenz, H., Sehested, E., & Grevle, I. S. (2002). Influence of boar and semen parameters on motility and acrosome integrity in liquid boar semen stored for five days. Acta Veterinaria Scandinavica, 43 (1), 49–55. doi: 10.1186/1751-0147-43-49.

Kummer, A., Gaggini, T., Bernardi, M., Mcmanus, C., Gonçales, E., Wentz, I. & Bortolozzo, F. (2013). Multivariate Analyses for Determining the Association of Field Porcine Fertility with Sperm Motion Traits Analysed by Computer-Assisted Semen Analysis and With Sperm Morphology. Reproduction in Domestic Animals, 48, 747-754. doi: 10.1111/rda.12155.

Lima, D. M. A., Pinho, R. O., Siqueira, J. B., Shiomi, H. H., Costa, E. V., Vergara, J. C. M., Campos, C. F., Lopes, P. S., Facioni Guimarães, S. E. & Guimarães, J. D. (2015). Correlation of sperm parameters with fertility in two commercial pig lines. Acta Scientiae Veterinariae, 43, 1-6. Retrieved May 5, 2019, from http://www.ufrgs.br/actavet/43/PUB%201290.pdf.

Marques, D. B. D., Lopes, M. S., Broekhuijse, M. L. W. J., Guimarães, S. E. F., Knol, E. F., Bastiaansen, J. W. M., Silva, F. F. & Lopes, P. S. (2017). Genetic parameters for semen quality and quantity traits in five pig lines. Journal of Animal Science, 95 (10), 4251-4259. doi: 10.2527/jas2017.1683.

Matabane, M.B., Thomas, R., Netshirovha, T.R., Tsatsimpe, M., Ng’ambi, J.W., Nephawe, K.A. & Nedambale, T.L. (2017). Relationship between sperm plasma membrane integrity and morphology and fertility following artificial insemination. South African Journal of Animal Science, 47 (1), 102-106. doi: 10.4314/sajas.v47i1.15.

Natumanya, R., Bamundaga, G., Kugonza, D. & Owiny, D. (2018). Reproductive performance of single and double artificial insemination protocol in swine. Bulletin of animal health and production in Africa, 66 (1), 143-157. Retrieved May 10, 2019, from Available in: https://www.researchgate.net/publication/323726735.

Niyiragira V., Rugira K. D. & Hirwa C. D. (2018). Success drivers of pig artificial insemination based on imported fresh semen. International Journal of Livestock Production, 9 (6), 102-107. doi: 10.5897/IJLP2018.0467.

Pereira, A. S., Shitsuka, D. M., Parreira, F. J., Shitsuka, R. (2018). Metodologia do trabalho científico [e-book]. Santa Maria, RS: UFSM, NTE. Retrieved April 2, 2020, from https://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1.

Petrocelli, H., Batista, C. & Gosálvez, J. (2015). Seasonal variation in sperm characteristics of boars in southern Uruguay. Revista Brasileira de Zootecnia, 44 (1), 1-7. doi: 10.1590/S1806-92902015000100001.

Popwell, J. M. & Flowers, L. W. (2004). Variability in relationships between semen quality and estimates of in vivo and in vitro fertility in boars. Animal Reproduction Science, 81 (1-2), 97-113. doi: 10.1016/j.anireprosci.2003.08.007.

R Core Team. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Viena, Áustria (2013). Retrieved Mar 3, 2019, from http://www.R-project.org.

Ruiz-Sánchez, A. L., O´Donoghue, R., Novak, S., Dyck, M. K., Cosgrove, J. R., Dixon, W. T. & Foxcroft, G. R. (2006). The predictive value of routine semen evaluation and IVF technology for determining relative boar fertility. Theriogenology, 66 (4), 736-748. doi: 10.1016/j.theriogenology.2005.12.012.

Soede, N. M. (1993). Boar stimuli around insemination affect reproductive processes in pigs: A review. Animal Reproduction Science, 32 (1-2), 107–125. doi: 10.1016/0378-4320(93)90062-V.

Soede, N. M., Noordhuizen, J. P. & Kemp, B. (1992). The duration of ovulation in pigs, studied by transrectal ultrasonography is not related to early embryonic diversity. Theriogenology, 38 (4), 653–666. doi: 10.1016/0093-691x(92)90028-p.

Tremoen, N. H, Gaustad, A. H., Andersen-Ranberg, I., Van Son, M., Zeremichael, T. T., Frydenlund, K., Grindflek, E., Våge, D. I. & Myromslien, F. D. (2018). Relationship between sperm motility characteristics and ATP concentrations, and association with fertility in two different pig breeds. Animal Reproduction Science, 193, 226-234. doi: 10.1016/j.anireprosci.2018.04.075.

Tsakmakidis, I.A., Lymberopoulos, A.G. & Khalifa, T.A.A. (2010). Relationship between sperm quality traits and field-fertility of porcine semen. Journal of Veterinary Science, 11(2), 151-154. Doi: http://dx.doi.org/10.4142/jvs.2010.11.2.151.

Vyt, P., Maes, D., Quinten, C., Rijsselaere, T., Deley, W., Aarts, M., De Kruif, A. & Van Soom, A. (2008). Detailed motility evaluation of boar semen and its predictive value for reproductive performance in sows. Vlaams Diergeneeskundig. Tijdschrift, 77 (5), 291-298. Retrieved Apr 20, 2019, from https://biblio.ugent.be/publication/746864/file/824717.pdf.




DOI: http://dx.doi.org/10.33448/rsd-v9i6.3389

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